balanced salt solution hbss (Thermo Fisher)

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Thermo Fisher balanced salt solution hbss
Balanced Salt Solution Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/balanced+salt+solution+hbss+buffer/PHOSPHATE+BUFFERED+SALINE+PBS/pm41915198-29-22-54
Average 99 stars, based on 1 article reviews

balanced salt solution hbss - by Bioz Stars, 2026-10

99/100 stars

Images

Imaging:

Article Title: Microglial MS4A4A Protects against Epileptic Seizures in Alzheimer's Disease
Article Snippet: .. Calcium Indicator Rhod‐2 for Calcium Imaging : Cultured primary microglia were washed twice with Hanks’ balanced salt solution (HBSS) buffer (1×HBSS with 1.26 × 10 −3 m Ca 2+ and 10 × 10 −3 m HEPES) before being loaded with 2 × 10 −6 m calcium indicator Rhod‐2 acetoxymethyl ester (R1245MP, Thermo Fisher Scientific) for 30 min at 37 °C. ..

Cell Culture:

Transfection:

Article Title: Zaltoprofen and its novel analogues exhibit dual targeting of COX-2 and PPAR-γ, providing a strategy to alleviate lipopolysaccharide - induced acute lung injury.
Article Snippet: .. CHO cells transfected with wild-type and mutant vectors of COX-2 were digested with trypsin 0.25% (w/v) (Hyclone, USA), and then collected by centrifugation at 1000 rpm for 5 min. CHO cells were washed with Hank’s balanced salt solution (HBSS) buffer (Thermo Fisher, USA) once, and resuspended in HBSS buffer (2.0×105 cells/mL). .. CHO cells were seeded in 48-well plates at 500 μL per well (1.0×105 cells/well), and incubated with the test compound (final concentration 100 μM ZPF, M2, M3 and M5) or dimethyl sulfoxide (DMSO) (Solarbio, China) for 15 min at 37°C before challenged with arachidonic acid.

Mutagenesis:

Centrifugation:

Concentration Assay:

Article Title: 3D Digital Light Processing Bioprinting and In Situ Differentiation of hiPSCs for Engineering Cardiac Tissue.
Article Snippet: The lack of physiologically relevant, reliable, scalable human cardiac tissue models remains a major barrier in cardiovascular disease research and drug development.. Conventional 2D culture systems and animal models fail to accurately recapitulate the complex structure and function of the human myocardium, while current 3D strategies utilizing pre-differentiated cells face challenges in cell survival, integration, and tissue maturation.. To address this critical gap, we developed FiBGel, a photocrosslinkable, choline bio-ionic liquid (BIL)-functionalized cold-water fish gelatin methacryloyl (GelMA), optimized for direct 3D bioprinting of undifferentiated human induced pluripotent stem cells (hiPSCs) and subsequent in situ cardiac differentiation.

Dispersion:

Immunofluorescence:

Article Title: Lysosomal damage is a therapeutic target in Duchenne muscular dystrophy
Article Snippet: Lysotracker Red DND (Invitrogen, L7528) was loaded in the media at a final concentration of 75 nM for 30 min at 37°C before cell fixation and imagination. .. For immunofluorescence, cells were briefly washed two times with Hanks’ balanced salt solution (HBSS) buffer (calcium and magnesium, Gibco), fixed with a 4% ice-cold paraformaldehyde (PFA) solution (Thermo Fisher Scientific, ref. 28906) for 15 min, then washed three times with PBS, and permeabilized for 10 min with 0.25% Triton X-100 (Sigma-Aldrich). ..

Review